- Serial cloner delete features how to#
- Serial cloner delete features install#
- Serial cloner delete features serial#
- Serial cloner delete features software#
A shortcut (S) to quickly show or mask Features (in the Features tab of Sequence Windows) A toggle button to highlight differences in aligned proteins Display Features in Protein Align (Local Align) Inherit Features when copying/Pasting Protein sequences Non formatted copy of Protein sequence (Copy More… Menu) A rapid switch between a DNA and Protein Align window Inherit Features when reverse-translating a DNA sequence Scan for peptide Features in Protein Sequences A button/menu to choose particular sites to be displayed in Restriction maps. An option in the Restriction Map window to only translate nucleotides that are in uppercase An option in the Preferences to use the Codon code from Codon Usage tables as a Genetic code used for translation An option in the Preferences to select pK value tables to use for pI estimation MacOSX version now distributed as a signed application (Mountain Lion Gatekeeper compatibility) Estimates Isoelectric point of protein sequences (DNA and Protein Sequence Window) Insertion point position when editing a protein sequence Error in definition of PreScission site
Serial cloner delete features install#
Slow down under certain Windows install due to autosaving Bug in "show info" toggle under Windows and Linux Detection of overlapping Primers in PCR a preference to limit or not the width of Seq & Prot windows (see the available contextual help window to get more details on new functions)
It also allows to generate sense and antisense sequences to be obtained after bi-sulfite conversion.
Serial cloner delete features serial#
Serial Cloner 2.5 now allows to import codon frequency tables from the internet using a Web interface. It is also posible to send directly BLAST request at the NCBI and obtain the result inside a Web interface.
Serial cloner delete features how to#
You will also find a Restriction Enzyme library management interface, Additional tools, like a web browser for direct import of NCBI and EMBL entries, a virtual cutter to prepare restriction analysis or a silent restriction map generator to find how to introduce restriction sites without modifying the translated peptide are also provided. Features are now visible when aligning locally. Finally, Serial Cloner provides an interface to align two sequences using a local algorithm or the BLAST2Seq NCBI server. An additional interface allows easy Gateway(tm) cloning for both BP and LR reactions. Just select, blunt if you need, and click the Ligate button. Finally, you can assemble fragments, obtained by PCR, adaptor/shRNA synthesis or simply by graphically selecting fragments between restriction sites. shRNA constructions based on pre-defined scaffolds are also automated. Many of the program's tools will only be familiar to scientists and academics but there's also a web browser for the direct import of NCBI and EMBL entries.PCR-based fragment or synthetic adaptors.
Obviously, the main aim of the program is to provide an easy way to clone DNA - a task which it does very well and mainly just using a graphical interface. You can numerically select fragments and carry out calculations of peptides which I have no idea about but certainly look impressive. All the tools you need to analyze and manipulate your DNA sequences are available in a very simple and easy to read all-in-one-window. The program includes a whole host of mapping options such as genetic maps, fragment viewers and a virtual PCR.
Serial cloner delete features software#
It is therefore an extremely powerful piece of software that will only be of interests to scientists and science-biology students. Serial Cloner is an application that allows you to read and write files DNA sequences that conform to the FASTA and pDRAW32 format. Until now, it reading and writing it was a very specialised field but this program intends to bring it to the masses. DNA is the complex code that makes up the lifeform in all of us.
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